Preguntas Frecuentes

How does the Universal Test plate analysis determine the pass/fail criteria for accuracy and repeatability?

Your Universal Test Plate comes with a tri-fold paper listing absorbance values at different wavelengths for your plate. (example below) These will be referred to as the "target" values.

For Accuracy measurements, the measurement on your instrument has to lie within specified limits of your target value for that wavelength as follows:

(Target – error) < measurement < (Target + error)

Where error = 1% + 0.01 + instrument specification

The instrument specification is found in the "Specifications" section of your Operator's Manual. It depends on factors such as the read type and plate type and OD of the measurement.

Example: For a Synergy HT, the accuracy specification according to the Operator's Manual in the range of 0.000-2.000 OD for a normal-mode read of a 96-well plate is + 1% + 0.010 OD The total error in this case will be + 2% + 0.020 (the sum of the errors of the instrument and the plate). The target value (from the trifold plate insert) is 0.149 for this example, so the error would be (0.149* 2%) + 0.020 = 0.023.

The passing range would be 0.149 + 0.023, or 0.126 < measurement < 0.172

For Repeatability measurements, you do not use the target value listed on the sheet that came with the plate. For these calculations, the reference is the first reading you make compared to a subsequent reading based on the specifications of your instrument.

Example: For a Synergy HT, the repeatability specification according to the Operator's Manual for a normal-mode read of a 96-well plate in the range of 2.00-3.00 OD is + 3% + 0.005.

For a first read of 2.676, the error would be (2.676 * 3%) + 0.005 = 0.085. The second reading must lie in the range of 2.676 + 0.085, or 2.591 < second measurement < 2.761

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