Cell Viability/Toxicity

Cell ViabilityTypically, cell viability or cytotoxicity assays assess the integrity of the cell’s plasma membrane. Fluorescent probes that undergo large fluorescence quantum yield increases as they intercalate into nuclear DNA can be engineered to be plasma membrane permeable or non-permeable. The use of these two probes is the basis of classic “LIVE/DEAD” assays. Alternatively only a membrane impermeable fluorometric dye can be used in a live cell kinetic assay such that toxicity can be measured in real time. Gen5 software fully automates this analysis, providing quantitative data through counting cells based on nuclear staining.