Genotoxicity refers to the destructive effects of mutagens such as high energy radiation and chemicals on cellular nucleic acids, particularly nuclear DNA. Single cell gel electrophoresis, or the comet assay, can be used to probe DNA fracture by mutagens and the g-H2AX immunofluorescence assay can used to quantify DNA double strand breaks. Each method has its challenges with respect to image analysis, but Gen5™ 3.0 has the ability to quantify either the %DNA in the comet tail or comet tail moment; or count g-H2AX foci to determine levels of genotoxicity.
- Automated Imaging and Analysis of a Novel Comet Assay to Enable High Throughput Genotoxicity Testing
- Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis
- Automated Imaging and Dual-Mask Analysis of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis
Visual Abstracts (.mp4):
BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microscopy workflow of a specific application.
- Lionheart FX Automated Microscope
- Lionheart LX Automated Microscope
- BioSpa Live Cell Imaging System
- Cytation 5 Cell Imaging Multi-Mode Reader
- Cytation 1 Cell Imaging Multi-Mode Reader
Learn more about Genotoxicity applications - search our Application Notes and technical documentation in Resources.