Congratulations to our 2018 winners
1st
2nd
3rd
Nicholas Cejda
Oklahoma Medical Research Foundation
Additional credit: David Forsthoefel, PhD, Sarah Guadiana, PhD.
Description: Planarian intestine, 4x, GFP TxRed and DAPI Montage, Two intestine genes are labeled by In Situ Hybridization in Schmidtea mediterranea, a new model organism studied for its astounding regenerative ability. When amputated, this intestine will completely regenerate in about two weeks, along with a new brain, eyes, muscles, pharnyx, gonads, skin, and more!
Image captured with: Lionheart FX
Dario Siniscalco
University of Campania
Additional credit: Jason Collier
Description: MAP2 (red) and GFAP (green) staining on mesenchymal stem cells in vitro neural differentiation induced; magnification 40X.
Image captured with: Cytation 3
Amy Abell
University of Memphis
Additional credit: Noha Shendy, R. Jackson Mobley and Lilly He
Description: Trophoblast stem cells expressing differential Ki67 staining.
The image was taken at 40X dry magnification using Z-stack imaging mode. In red is the actin stain, in green is Ki67 stain (a proliferation marker) and in blue is nuclei stain. The differential Ki67 staining in the nucleus is the indicator of cell cycle stage of the cell.
Image captured with: Lionheart FX
Honorable Mentions
David Burk, Pennington Biomedical Center
Description: Mouse e7.5 implantation site imaged at 10x. Red is anti-Complement C3, blue are DAPI-stained nuclei, and green is tissue autofluorescence.
Image captured with: Lionheart FX
Kexiao Zheng, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences
Description: Neural crest-derived stem cells in a fluorescent labeled heart tissue section.
Image captured with: Cytation 3
Miarasa Steele, Case Western Reserve University
Description: A single mouse small intestine organoid embedded in a MatiriGel dome, stained with fluorescent dyes, and imaged at 10X using manual mode. This organoid was allowed to proliferate for two weeks without passaging, resulting in the highly budded structure. Colors were inverted in Photoshop for aesthetic purposes.
Image captured with: Lionheart FX
Nicola Alessio, Universita della Campania
Description: ICC for GFAP (green), MAP2 (red) and DAPI (blue) in Neural stem Cell from Mice after differentiation, (10x10 300dpi, 40x magnification).
Image captured with: Cytation 3
Sean Naughton, Augusta University
Description: Large Network of Cortical Neurons ( primary cortical neurons, 20x, stitched together from 24images, Beta Tubulin in Red, Synapsin in Green, Nuclei in blue).
Image captured with: Cytation 3
Shaoxun Wang, University of Mississippi Medical Center
Description: F-actin and G-actin co-localization in rat middle cerebral artery. The picture was taken using Z-stack and generated by Z-projection.
Image captured with: Lionheart FX
Shimara Gunawardana, South Dakota State University
Description: Filamentous-actin (Alexa Fluor® 488 and Dylightâ Phalloidin) and Globular-actin (Alexa Fluor® 594 Deoxyribonuclease-I) staining of Hs578T triple negative breast cancer cell line using a 40X objective in manual mode. G-actin monomers combine to form F-actin bundles that mediate migration of breast cancer cells.
Image captured with: Cytation 3
Ying-Hsuan Shih, University of California- Santa Barbara
Description: Wound healing assay, testing cell regeneration of human primary retinal pigmented epithelium using immunostaining (blue: hoechst33342, green: phalloidin, red: ki-67 ). Images were taken using cytation5, 20x montage.
Image captured with: Cytation 5