product design

3D Cell Culture

0 uM IT1t Day 5 BF-GFP-RFP Cellular AnalysisOver the last decade, a central focus of drug discovery efforts has been the incorporation of in vitro testing models that mimic in vivo conditions found within and around the target. This has led to the development of multiple methods to culture cells in a three-dimensional (3D) format instead of on the bottom of a microplate well in a two-dimensional (2D) format. Some of the most commonly incorporated technologies to create the desired spheroids or tumoroids include, but are not limited to polymeric and biological scaffolds, ultra-low attachment, hanging drop, and magnetic bioprinting.

3D cell culture is now used diverse application areas such as:

  • Oncology
  • Drug and Environmental Toxicology
  • Cosmetics
  • Stem Cell Research

Many procedures using cells cultured in 3D are performed in a variety of microplate types ranging from 6- to 1536-well format, and are commonly analyzed using microplate reader optics or digital widefield microscopy.

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BioTek has developed a suite of application notes describing adaptation of these diverse processes using its line of liquid handling, detection, and imaging instrumentation


Application Notes:


Learn more about 3D Cell Culture applications - search our Application Notes and technical documentation in Resources.