Live Cell Assays

Kinetic live cell assays provide real-time data of cellular responses. BioTek instruments enable a wide variety of live cell applications addressing diverse biological processes, ranging in timescales from milliseconds to multiple days.

Studies involving fast-acting cellular responses, including Ca²⁺ mobilization in response to GPCR activation, require the ability to capture events with sub-second resolution. Available inline reagent injectors and rapid image capture rates support uninterrupted monitoring of cells and provide detailed characterization of events.

Quantifying cell viability and growth rates is a crucial aspect of biological research and therapeutic drug development. Complete environmental control, including CO₂, O₂, temperature, and humidity, ensures optimal conditions for conducting long-term kinetic cell viability and proliferation assays.

Fibroblast Uninhibited Wound Healing

The following application notes provide a sampling of the live cell assays that were optimized on BioTek microplate readers and automated imaging systems for robust and reproducible results.

Application Notes:

• Characterizing Calcium Mobilization using Kinetic Live Cell Imaging
• Kinetic Proliferation Assay Using Label-Free Cell Counting
• Live Cell Hypoxia Determination in a 3D Spheroid Model