Recursos Técnicos - Boletines de Aplicación
BioTek Resources for Agilent MitoXpress® Xtra Oxygen Consumption AssayDescargar
June 27, 2018
The Agilent MitoXpress® Xtra Oxygen Consumption Assay allows real-time measurement of oxygen consumption of whole cells or isolated mitochondria. BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation™ 1, Cytation 5, Synergy™ H1, or Synergy Neo2 instruments equipped with TRF capability.
Figure 1 - Example Data (Cytation 5): Lifetime detection of extracellular oxygen consumption in HepG2 cells (left). Slope is calculated from the linear portion of the curves to calculate oxygen consumption rates (OCR) of various cell treatments (right).
Figure 2. A summary of the AgilentpHX.prt procedure, plate layout, and data reduction steps as shown from within Gen5 v3.04 software.
Figure 3. Top level implementation steps for the Agilent MitoXpress Xtra – Oxygen Consumption Assay with the BioTek Cytation 1, Cytation 5, Synergy H1, or Synergy Neo2 instruments. The toolkit is designed for models equipped with Time Resolved Fluorescence (TRF) capability.