Recursos Técnicos - Boletines de Aplicación

BioTek Toolkit for Agilent MitoXpress® Xtra Oxygen Consumption Assay

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Related Products: Lector Multi-Modal y de Captura de Imágenes Cytation 1, Lector Multi-Modal con Captura de Imágenes Celulares Cytation 5, Lector Multi-Modal Híbrido Synergy H1

May 02, 2018

Red arrowRelated Sample File: AgilentX02

Introduction

The Agilent MitoXpress® Xtra Oxygen Consumption Assay allows real-time measurement of oxygen consumption of whole cells or isolated mitochondria. BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation™ 1, Cytation™ 5, or Synergy™ H1 instruments equipped with TRF capability.

 

 

8040588 cube
Filter Cube: P/N 8040587 FLTR BLK MITOXPRESS XTRA ASBY

 

Gen5
Gen5 v3.04 Data Analysis Protocol: AgilentXO2.prt
Note: The User Tutorial and Data Analysis Protocol are compatible with Gen5 v3.04 software. Existing Gen5 users can upgrade their software for free at: https://btresource.force.com/CRC/s/

 

Lifetime detection of extracellular oxygen consumption

Example Data (Cytation 5): Lifetime detection of extracellular oxygen consumption in HepG2 cells (left). Slope is calculated from the linear portion of the curves (10-80 mins) to calculate oxygen consumption rates of various cell treatments (right).

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