Recursos Técnicos - Boletines de Aplicación
BioTek Toolkit for Agilent pH-Xtra™ Glycolysis AssayDescargar
May 02, 2018
Related Sample File: AgilentXpH
The Agilent pH-Xtra™ Glycolysis Assay allows direct, real-time, kinetic analysis of extracellular acidification rates (ECA/ECAR). BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation™ 1, Cytation™ 5, or Synergy™ H1 instruments equipped with TRF capability.
Example data of a glucose oxidase titration performed on the Synergy H1 illustrates the detection principle of the Agilent pH probe: higher lifetime signals (left) are proportional to increased acidification (middle). H+ and ECAR values can be calculated using Gen5 software (right).