Recursos Técnicos - Posters Científicos

Homogeneous Cell-Based Signal Transduction Assays using a Novel 3-D Cell Culture Matrix


October 05, 2010


Authors: A. Rieger, G. Prescott, J. Wetterwald, P. Banks, B. Larson, BioTek Instruments, Inc., Winooski, Vermont; B. Justice, Global Cell Solutions, Inc., Charlottesville, VA; J. March, Hamilton Robotics, Inc., Reno, NV


Global Cell Solutions     Hamilton




  • Mammalian Target of Rapamycin (mTOR) protein kinase represents a key target in today’s drug discovery efforts due to its point of convergence for multiple cell signaling pathways
  • The AlphaScreen® SureFire® p-p70 S6 Kinase(Thr389) Assay provides an easy to use, and sensitive method to assay mTOR activity
  • Performing assays using cells grown on the GEM matrix simplifies the experimental process and provides results that are equivalent to control data generated with cells cultured using standard 2D methods
  • Validation data, as well as insulin receptor agonist/antagonist data, demonstrate how the combination of assay and instrumentation can provide relevant data to measure activity of this important drug target




Utilizamos cookies para proporcionar a los visitantes de nuestro sitio web, la mejor experiencia posible. Si continúa sin cambiar la configuración de su navegador, asumiremos que está de acuerdo en recibir todas las cookies del sitio web de BioTek. Para obtener más información sobre cómo utilizamos las cookies o cómo bloquear las cookies,  visite nuestra sección de política de cookies.