Recursos Técnicos - Posters Científicos

Utility of a Sensitive, Fluorescence-Based Assay for the Detection of Histone Deacetylase 6 (HDAC6) Activity

Descargar

March 31, 2011

 

Authors: Brad Larson & Peter Banks, BioTek Instruments, Inc., Winooski, Vermont; Diana Hulboy, Kara Cannon & Wayne Patton, Enzo Life Sciences, Farmingdale, New York

 Enzo Life Sciences Logo

Overview

 

Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from ε-N-acetyl lysine amino acids on histones and other proteins. The removal of acetyl groups serves to increase the positive charge of histone tails, encouraging binding between histones and the DNA backbone, and preventing transcription. Because of this role, HDACs have been implicated in a variety of human diseases, including cancer; making them an increasingly popular target for drug discovery research. Here we demonstrate an application to monitor the deacetylation activity of the HDAC Class II enzyme, HDAC6. Validation and pharmacology results demonstrate how the fluorescent assay technology and microplate reader can be used together to provide a relevant system to assess enzyme kinetics and inhibition

 

 

link

Utilizamos cookies para proporcionar a los visitantes de nuestro sitio web, la mejor experiencia posible. Si continúa sin cambiar la configuración de su navegador, asumiremos que está de acuerdo en recibir todas las cookies del sitio web de BioTek. Para obtener más información sobre cómo utilizamos las cookies o cómo bloquear las cookies,  visite nuestra sección de política de cookies.