Recursos Técnicos - Posters Científicos

Automated 3D Spheroid Culture Media Exchange Using a Novel MultiFlo™ FX Accessory


Related Products: Dispensador Multi-Modal MultiFlo FX

February 06, 2019

Authors: Peter Banks, Brad Larson, BioTek Instruments, Inc., Winooski, VT, USA


Spheroidal 3D cellular structures are a mainstay in many research areas, including oncology and toxicology. Culturing cells in 3D provides a more in vivo-like environment, allowing cells to maintain high viability when cultured for extended time periods. To maintain the highest levels of viability within untreated cells and ensure that observed effects are solely from treatment, media exchanges and re-dosing are necessary throughout the experiment, particularly in vitro tests lasting weeks. Media exchanges with cell models that do not rely on adherence to labware can be daunting if performed by hand, even when performed on a single plate. Multichannel pipettes must remove and dispense media at an extremely slow rate, and care must be taken to keep pipette tips away from the actual spheroids.

Through incorporation of the AMX™ Automated Media Exchange module on the MultiFlo™ FX Multi-Mode Dispenser, risk of accidental spheroid removal from wells is eliminated. Spent media is automatically removed and replaced with fresh media only, or fresh media containing treatment concentrations.

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