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Recursos Técnicos - Resúmenes Visuales

Kinetic Characterization of Gs- and Gi-Dependent Regulation of cAMP

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February 16, 2018

Red arrow Related Application: Cell Proliferation

An expressed cAMP fluorescent biosensor (cADDis, Montana Molecular) is kinetically monitored in live cells to characterize the cAMP-dependent signaling pathway following G protein coupled receptor (GPCR) activation. Dual in-line dispense tips and rapid image capture rates support sequential addition of Gs- and Gi-coupled receptor agonists for detailed analysis of cAMP signaling events.

Kinetic Characterization