Quantitative Intracellular and Intranuclear Spot Analysis Using Digital Microscopy

Presenters: Sarah Beckman, Principal Scientist and Wendy Goodrich, Applications Scientist, BioTek Instruments

Phenotypic cellular analysis is a critical area of biological research. Numerous applications require quantifying the number of  “spots” per cell, including DNA damage, autophagosomes, endosomes, and mitochondria. These evaluations are often done by hand, which is highly subjective and can be tedious. In this webinar we will describe using an automated image based approach for rapid, objective quantification of various assays that use object spot counting analysis including autophagy, mitochondria membrane potential, genotoxicity, and others.