Recursos Técnicos - Webinars
ELISA: Best Practices and Troubleshooting - On Demand
Related Products: Lavador 405 LS, Lavador 405 TS, Lavador 50 TS, Lector de Absorbancia 800 TS, Incubador Automatizado BioSpa 8, Sistema de Captura de Imágenes para Células Vivas BioSpa, BioStack , Lector Multi-Modal Híbrido Cytation , Lavador Dispensador EL406, Lavador ELx405 Select Deep Well, Lector de Absorbancia ELx808, Software Gen5 Características para Detección, Software Liquid Handling Control, Lector Multi-Modal Híbrido Synergy H1, Synergy HTX, Lector Híbrido Multi-Modal Synergy Neo2
September 20, 2017
Presenter: Dr. Paul Held - Applications Laboratory Manager, BioTek Instruments
Enzyme-linked immunosorbent assay (ELISA) is an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process. The reaction uses a solid surface, typically a microplate, to immobilize the analyte. Once immobilized, unwanted materials can be removed by washing and the remaining analyte quantitated. The assay technology uses antibodies in conjunction with enzyme-catalyzed amplification to provide high specificity and high sensitivity. Here we will describe the basic process steps of ELISA, best laboratory practices along with troubleshooting tips for common problems. In addition, some of the devices and instrumentation used with this assay technology will be described.